Laboratory Protocols

Journal Club
Light Regulation of Plant Growth and Development

PMCB Class

Directions to Lab at UF

Research Group

Building LED Light Sources for Plant Research

Spectra of Light Sources Used in Published Experiments

 Lab Resource Page

Photography

Stock Antibiotic Solutions and Working Concentrations

Stock solution Working concentration: Strict control Weak control mg/ml:           µg/ml dilution µg/ml dilution Ampicillin 50 (H2O*) -20oC 20 0.4 µl/ml 60-125 1.2-2.5 µl/ml Carbenicillin 50 (H2O, 50%EtOH) -20oC 20 0.4 µl/ml 60-200 1.2-4 µl/ml Chloramphenicol 34 (EtOH, MetOH) -20oC 25 0.7 µl/ml 170 5 µl/ml D-cycloserine* 10 (0.1M NaP pH8.0) +4oC 200 20 µl/ml     Gentamycin 10 (H2O) -20oC 15 1.5 µl/ml     Hygromycin B 10 (H2O) -20oC 10 1 µl/ml 400 40 µl/ml Kanamicin 50 (H2O) +4oC- -20oC 10 0.2 µl/ml 50 1 µl/ml Kasugamycin 10 (H2O) -20oC 1000 100 µl/ml     Nalidixic acid 5 (H2O, pH to 11 /NaOH) -20oC 15 3.0 µl/ml     Neomycin 10 (H2O) -20oC 100 10 µl/ml 800 80 µl/ml Rifampicin** 34 (MetOH) -20oC 150 4.4 µl/ml     Spectinomycin 10 (H2O) -20oC 100 10 µl/ml     Streptomicin 50 (H2O) -20oC 10 0.2 µl/ml 50 1 µl/ml Tetracycline*** 5 - 12.5 (EtOH, 70%EtOH) -20oC 10 2 µl/ml 50 100 10 µl/ml 20 µl/ml